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Does it matter which PCI-E slot a card is installed?

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I mean the slot 1 and 2?
 
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Some boards tend to make the Primary slot a full 16x lane, so thats what I use. In theory though any PCI-E lane works.
 
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Some boards tend to make the Primary slot a full 16x lane, so thats what I use. In theory though any PCI-E lane works.

I have a X58 board so i believe both are full x16 lanes
 
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Yeah it should be fine. :)

Why the questions though?
 
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Ahh, the 295's then.
 
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newfellow

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Well, I don't think in performance sense, but cooling sense it's important at least cases which mounts the PSU on bottom.

Here's poor man(Like I am) GPU mod:
I did saw off half the blastic parts(all black out to second screw holes) & added AS5 to/from stock GTX 9800 sometime ago. dropped it on PCI-E slot 2 right on top of PSU 120mm fan and temps went from ~60c/~85c (stock idle/load) to ~40c/~65c (still same stock cooling, with little modification, idle/load) and fan spins like 36-38% at max (damn NVIDIA Bios minimum limits ;P ).

So, sometimes it means a lot.
 
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